DUBs Family


Protein ubiqutinylation, the covalent post-translational addition of ubiquitin, and deubiquitinylation is a dynamic and reversible process, orchestrated by the concerted effort of the ubiquitin ligase and deubiquitin peptidase (DUBs) families. Ubiquitinylation is critical in regulating protein degradation, protein-protein interactions, cellular localization, and enzymatic activity - dictating numerous important cellular processes. 


DUBs are a family of peptidases responsible for cleaving or editing these ubiquitin chains, thereby rescuing proteins from proteosomal degradation and restoring intracellular signaling cascades. With 6 distinct classes of DUBs, over 100 diverse family members total, categorized by their well characterized catalytic domains, DUBs serve as attractive therapeutic targets. Dysregulation of DUB activity has been implicated in a variety of human disease states, most commonly cancer. Recent advancements have contributed to the renewed interest in the therapeutic potential of pharmacologically modulating DUBs. 

 

Figure 1. DUB and Setrin/SUMO specific protease(SENP) phylogenetic tree. All 6 classes of DUBs are represented here: ubiquitin-specific proteases (USP), ubiquitin carboxy-terminal hydrolases (UCH), Machado-Josephin domain-containing proteases (MJD), ovarian tumor proteases (OTU), motif-interacting with ubiquitin-containing novel DUB family (MINDY) and JAB1, MPN, MOV34 family (JAMM). 
from Harrigan, J. et al, Nature Reviews 2016.

 

 

 

The Biortus Protein Catalogue currently offers 67 DUBs expressed in multiple expression systems, with 3 structurally resolved and specific in-house biochemical assays for all 6 classes of DUBs readily available.

 

Catalog No.

Organism

Expression system

Tag

Sequence

BP12389-01A

Homo sapiens

E. coli

His

D2-R436

BP12432-14A

Homo sapiens

Insect cell

His/Flag

D2-K355

BP12774-01A

Homo sapiens

E. coli

His

S2-D188

BP13015-01A

Homo sapiens

E. coli

His

Q2-S481

BP12849-01A

Homo sapiens

E. coli

His

F2-V348

BP12839-01A

Homo sapiens

E. coli

His

Q2-A223

BP12423-01A

Homo sapiens

Insect cell

His

P2-K324

BP12769-00A

Homo sapiens

E. coli

No tag

D2-K212

BP12837-01A

Homo sapiens

E. coli

His

T2-K329

 

 

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For custom DUBs generation, please see Protein Generation or contact info@wuxibiortus.com.

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