E3 Ligase Family


Protein degradation is a regulated, multistep process. Ubiquitin is loaded onto E1-activating enzymes in an ATP-dependent manner and then transferred to E2-binding enzymes. The same E2 can bind to many E3 ligases, which in turn can ubiquitinate multiple target substrate proteins. E3 ligases bind specific substrate proteins based on substrate degron motifs.


E3 ubiquitin ligases play crucial roles in eukaryotic biology by promoting protein ubiquitination and degradation. Human genome encodes over 600 E3 ubiquitin ligases which could function in distinct cellular contexts, respond to different cellular signals, and process diverse protein targets. In recent years, various E3 ligases became pharmacological targets for developing drugs. Two novel strategies, proteolysis-targeting chimeras (PROTAC) and molecular glue make E3 ligase protein production necessary for structure-based drug design.


The Biortus Protein Catalogue currently offers over 100 of E3 ubiquitin ligases expressed in multiple expression systems, with several structurally resolved and assay developments ready.

 

Catalog No.

Organism

Expression system

Tag

Sequence

BRAP-H01A

Homo sapiens

E. coli

His

S2-K592

MDM4-H01B

Homo sapiens

E. coli

His

T2-A490

RNF4-H02D

Homo sapiens

E. coli

GST

G120-I190

RNF114-H02C

Homo sapiens

E. coli

GST

A2-Q228

SH3RF3-H01A

Homo sapiens

E. coli

His

L2-F882

TRIM44-H13B

Homo sapiens

Insect cell

His/Strep

A2-T344

XIAP-H02B

Homo sapiens

E. coli

GST

Y120-P260

cIAP1-H13C

Homo sapiens

Insect cell

His/Strep

M1-S618

SMURF2-H01A

Homo sapiens

E. coli

His

S2-E748

WWP2-H01A

Homo sapiens

E. coli

His

A2-E870

KLHL42-H13A

Homo sapiens

Insect cell

His/Strep

S2-T505

STUB1-H01A

Homo sapiens

E. coli

His

K2-Y303

CUL5-H13A

Homo sapiens

Insect cell

His/Strep

A2-A780

DCA16-H01A

Homo sapiens

Insect cell

His

G2-L216

MDM2-H01A

Homo sapiens

E. coli

His

S17-N111

RNF14-H01A

Homo sapiens

E. coli

His

S2-D474

NEDD4L-H01A

Homo sapiens

E. coli

His

A2-D975

DCAF15-H13B

Homo sapiens

Insect cell

His/Strep

A2-L600

 

 

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For custom E3 ligase protein generation, please see Protein Generation or contact info@wuxibiortus.com.

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