E3 ubiquitin-protein ligase
Catalog No. ：
Expression system ：
Symbol/Gene Name: WWP2
Alias: NEDD4-like E3 ubiquitin-protein ligase WWP2,Atrophin-1-interacting protein 2,AIP2,HECT-type E3 ubiquitin transferase WWP2,WW domain-containing protein 2
Background: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; in embryonic stem cells (ESCs) the ubiquitination is proposed to regulate POU5F1 protein level. Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation-induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation.
Construct: 6His-TEV-(GG)-WWP2(A2-E870 end)
Expression Host: E. coli
Supplied as sterile 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 5% glycerol, 1 mM DTT.
Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
< 0.01 EU per μg of the protein
Liquid. It is shipped out with blue ice.
Stability & Storage:
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.