ANKFY1-H13A

Family :

E3 ubiquitin-protein ligase

Catalog No. :

ANKFY1-H13A

Protein :

ANKFY1

UniProt :

Q9P2R3

Organism :

Homo sapiens

Expression system :

Insect cell

Tag :

His/Strep

Sequence :

A2-S1169

Assay :

Structure :

Inquiry :

Product Information

Symbol/Gene Name:  ANKFY1

Alias:  Rabankyrin-5,Rank-5,Ankyrin repeat and FYVE domain-containing protein 1,Ankyrin repeats hooked to a zinc finger motif

Uniprot: Q9P2R3

Background: Proposed effector of Rab5. Binds to phosphatidylinositol 3-phosphate (PI(3)P). Involved in homotypic early endosome fusion and to a lesser extent in heterotypic fusion of chlathrin-coated vesicles with early endosomes. Involved in macropinocytosis; the function is dependent on Rab5-GTP. Required for correct endosomal localization. Involved in the internalization and trafficking of activated tyrosine kinase receptors such as PDGFRB. Regulates the subcellular localization of the retromer complex in a EHD1-dependent manner. Involved in endosome-to-Golgi transport and biosynthetic transport to late endosomes and lysosomes indicative for a regulation of retromer complex-mediated retrograde transport.

Construct: 8His-StrepII-TEV-(GG)-ANKFY1(A2-S1169 end)

Expression Host: Insect cell

Purity: 90%

Formulation: 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol

Supplied as sterile

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20 to -80

Store it under sterile conditions at -20 to -80. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.