HACE1-H00A

Family :

E3 ubiquitin-protein ligase

Catalog No. :

HACE1-H00A

Protein :

HACE1

UniProt :

Q8IYU2

Organism :

Homo sapiens

Expression system :

E. coli

Tag :

No tag

Sequence :

E2-A909

Assay :

Structure :

Inquiry :

Symbol/Gene Name:  HACE1

Alias:  E3 ubiquitin-protein ligase HACE1,HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1,HECT-type E3 ubiquitin transferase HACE1

Uniprot: Q8IYU2

Background: E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB.

Construct: (GG)-HACE1(E2-A909 end)

Expression Host: E. coli

Purity: 85%

Formulation: 50mM Tris-HCl (pH 7.5), 200mM NaCl, 20% glycerol, 1mM DTT

Supplied as sterile

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20 to -80

Store it under sterile conditions at -20 to -80. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.