CHFR-H01A

Family :

E3 ubiquitin-protein ligase

Catalog No. :

CHFR-H01A

Protein :

CHFR

UniProt :

Q96EP1

Organism :

Homo sapiens

Expression system :

E. coli

Tag :

His

Sequence :

E2-N664

Assay :

Structure :

Inquiry :

Product Information

Symbol/Gene Name:  CHFR

Alias:  E3 ubiquitin-protein ligase CHFR,Checkpoint with forkhead and RING finger domains protein,RING finger protein 196,RING-type E3 ubiquitin transferase CHFR

Uniprot: Q96EP1

Background: E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.

Construct: 6His-TEV-(GG)-CHFR(E2-N664 end)

Expression Host: E. coli

Purity: 90%

Formulation: 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol

Supplied as sterile

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20 to -80

Store it under sterile conditions at -20 to -80. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.