SF3B3-H13A
Family :
E3 ubiquitin-protein ligase
Catalog No. :
SF3B3-H13A
Protein :
SF3B3
UniProt :
Q15393
Organism :
Homo sapiens
Expression system :
Insect cell
Tag :
His/Strep
Sequence :
F2-F1217
Assay :
Structure :
Inquiry :
是
Symbol/Gene Name: SF3B3
Alias: Splicing factor 3B subunit 3,Pre-mRNA-splicing factor SF3b 130 kDa subunit,SF3b130,STAF130,Spliceosome-associated protein 130,SAP 130
Uniprot: Q15393
Background: Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex, a constituent of the spliceosome (PubMed:10490618, PubMed:10882114, PubMed:27720643, PubMed:28781166). SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937). May also be involved in the assembly of the 'E' complex (PubMed:10882114). Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron (PubMed:15146077).
Construct: 8His-StrepII-TEV-(GG)-SF3B3(F2-F1217 end)
Expression Host: Insect cell
Purity: 90%
Formulation: 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
Supplied as sterile
Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Endotoxin:
< 0.01 EU per μg of the protein
Shipping:
Liquid. It is shipped out with blue ice.
Stability & Storage:
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Recombinant protein