FEN1-H13A

Family :

Protease and Enzyme

Catalog No. :

FEN1-H13A

Protein :

FEN1

UniProt :

P39748

Organism :

Homo sapiens

Expression system :

E. coli

Tag :

His/Strep

Sequence :

G2-K380

Assay :

Structure :

Inquiry :

Product Information

Symbol/Gene Name:  FEN1

Alias:  Flap endonuclease 1,FEN-1,DNase IV,Flap structure-specific endonuclease 1,Maturation factor 1,MF1,hFEN-1

Uniprot: P39748

Background: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

Construct: 6His-strepII-TEV-GG-FEN1(G2-K380 end)

Expression Host: E. coli

Purity: 90%

Formulation: 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol

Supplied as sterile

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20 to -80

Store it under sterile conditions at -20 to -80. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.