Protease and Enzyme
Catalog No. ：
Expression system ：
Symbol/Gene Name: hmp
Alias: 3-ketosteroid-9-alpha-monooxygenase, ferredoxin reductase component, 3-ketosteroid-9-alpha-hydroxylase, ferredoxin reductase component, KSH, Androsta-1, 4-diene-3, 17-dione 9-alpha-hydroxylase, Rieske-type oxygenase, RO
Background: Involved in the degradation of cholesterol. Catalyzes the introduction of a 9a-hydroxyl moiety into 1,4-androstadiene-3,17-dione (ADD) to yield the 9alpha-hydroxy-1,4-androstadiene-3,17-dione (9OHADD) intermediate which spontaneously form 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione (HSA) via the meta-cleavage of ring B with concomitant aromatization of ring A. KSH is also able to use 4-androstene-3,17-dione (AD), 3-oxo-23,24-bisnorcholesta-4-en-22-oate (4-BNC), 3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate(4-BNC), 3-oxo-23,24-bisnorcholesta-4-en-22-oyl-coenzyme A thioester (4-BNC-CoA) and 3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-coenzyme A thioester(4-BNC-CoA) as substrates.
Construct: 6His-TEV-GG-KSHB(M1-E358 end) (TB enzyme)
Expression Host: E. coli
Formulation: 50mM Tris-HCl (pH 7.5), 200mM NaCl, 20% glycerol
Supplied as sterile
Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
< 0.01 EU per μg of the protein
Liquid. It is shipped out with blue ice.
Stability & Storage:
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.