lipoxygenase-O41A

Family :

Protease and Enzyme

Catalog No. :

lipoxygenase-O41A

Protein :

lipoxygenase

UniProt :

Q8RNT4

Organism :

Pseudomonas aeruginosa

Expression system :

E. coli

Tag :

Flag/His

Sequence :

M1-I678

Assay :

Structure :

Inquiry :

Symbol/Gene Name:  lox

Alias:  Linoleate 9/13-lipoxygenase, Oleate 10S-lipoxygenase

Uniprot: Q8RNT4

Background: In presence of oxygen, converts linoleate into (9S)-hydroperoxy-10,12-octadecenoate (9HPOD), which spontaneously decomposes to the corresponding 9-hydroxy-10,12-octadecenoate (9HOD), and into 13-hydroperoxy-9,11-octadecenoate (13HPOD) which spontaneously decomposes to the corresponding 13-hydroxy-9,11-octadecenoate (13HOD). Also active on linolenate. To a lesser extent, is also able to convert oleate into (10S)-hydroperoxy-8E-octadecenoate, which spontaneously decomposes to the corresponding 10-hydroxy-8E-octadecenoate. Is almost not active on arachidonate.

Construct: Flag-6His-TEV-G-lipoxygenase(M1-I678 end)

Expression Host: E. coli

Purity: 90%

Formulation: 50mM Tris-HCl (pH 7.5), 200mM NaCl, 20% glycerol

Supplied as sterile

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20 to -80

Store it under sterile conditions at -20 to -80. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

 

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