UBA5-H00A

Family :

E1 ubiquitin-activating enzyme

Catalog No. :

UBA5-H00A

Protein :

UBA5

UniProt :

Q9GZZ9

Organism :

Homo sapiens

Expression system :

Insect cell

Tag :

No tag

Sequence :

A2-M404

Assay :

Structure :

Inquiry :

Symbol/Gene Name:  UBA5

Alias:  Ubiquitin-like modifier-activating enzyme 5,Ubiquitin-activating enzyme 5,ThiFP1,UFM1-activating enzyme,Ubiquitin-activating enzyme E1 domain-containing protein 1

Uniprot: Q9GZZ9

Background: E1-like enzyme which specifically catalyzes the first step in ufmylation (PubMed:15071506, PubMed:18442052, PubMed:25219498, PubMed:20368332, PubMed:27653677, PubMed:26929408, PubMed:27545674, PubMed:30412706, PubMed:27545681). Activates UFM1 by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a UFM1-E1 thioester and free AMP (PubMed:20368332, PubMed:27653677, PubMed:26929408, PubMed:30412706). Activates UFM1 via a trans-binding mechanism, in which UFM1 interacts with distinct sites in both subunits of the UBA5 homodimer (PubMed:27653677). Trans-binding also promotes stabilization of the UBA5 homodimer, and enhances ATP-binding (PubMed:29295865). Transfer of UFM1 from UBA5 to the E2-like enzyme UFC1 also takes place using a trans mechanism (PubMed:27653677). Ufmylation is involved in reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress (PubMed:32160526). Ufmylation is essential for erythroid differentiation of both megakaryocytes and erythrocytes (By similarity).

Construct: (GG)-UBA5(A2-M404 end)

Expression Host: Insect cell

Purity: 80%

Formulation: 50mM Tris-HCl (pH 7.5), 200mM NaCl, 20% glycerol, 1mM DTT.

Supplied as sterile

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20 to -80

Store it under sterile conditions at -20 to -80. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.