UBE2A-H13A

Family :

E2 ubiquitin-conjugating enzyme

Catalog No. :

UBE2A-H13A

Protein :

UBE2A

UniProt :

P49459

Organism :

Homo sapiens

Expression system :

Insect cell

Tag :

His/Strep

Sequence :

S2-C152

Assay :

Structure :

Inquiry :

Symbol/Gene Name:  UBE2A

Alias:  Ubiquitin-conjugating enzyme E2 A,E2 ubiquitin-conjugating enzyme A,RAD6 homolog A,HR6A,hHR6A,Ubiquitin carrier protein A,Ubiquitin-protein ligase A

Uniprot: P49459

Background: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA.

Construct: 8His-StrepII-TEV-(GG)-UBE2A(S2-C152 end)

Expression Host: Insect cell

Purity: 90%

Formulation: 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol

Supplied as sterile

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20 to -80

Store it under sterile conditions at -20 to -80. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.