UBE2B-H00A

Family :

E2 ubiquitin-conjugating enzyme

Catalog No. :

UBE2B-H00A

Protein :

UBE2B

UniProt :

P63146

Organism :

Homo sapiens

Expression system :

Insect cell

Tag :

No tag

Sequence :

S2-S152

Assay :

Structure :

Inquiry :

Symbol/Gene Name:  UBE2B

Alias:  Ubiquitin-conjugating enzyme E2 B,E2 ubiquitin-conjugating enzyme B,RAD6 homolog B,HR6B,hHR6B,Ubiquitin carrier protein B,Ubiquitin-conjugating enzyme E2-17 kDa,Ubiquitin-protein ligase B

Uniprot: P63146

Background: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.

Construct: (GG)-UBE2B(S2-S152 end)

Expression Host: Insect cell

Purity: 85%

Formulation: 50mM Tris-HCl (pH 7.5), 200mM NaCl, 20% glycerol, 1mM DTT.

Supplied as sterile

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20 to -80

Store it under sterile conditions at -20 to -80. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.