E2 ubiquitin-conjugating enzyme
Catalog No. ：
Expression system ：
Symbol/Gene Name: UBE2F
Alias: NEDD8-conjugating enzyme UBE2F,NEDD8 carrier protein UBE2F,NEDD8 protein ligase UBE2F,NEDD8-conjugating enzyme 2,RING-type E3 NEDD8 transferase UBE2F,Ubiquitin-conjugating enzyme E2 F
Background: Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
Construct: 8His-StrepII-TEV-(GG)-UBE2F(L2-R185 end)
Expression Host: Insect cell
Formulation: 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
Supplied as sterile
Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
< 0.01 EU per μg of the protein
Liquid. It is shipped out with blue ice.
Stability & Storage:
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.