UBE2O-H13A

Family :

E2 ubiquitin-conjugating enzyme

Catalog No. :

UBE2O-H13A

Protein :

UBE2O

UniProt :

Q9C0C9

Organism :

Homo sapiens

Expression system :

Insect cell

Tag :

His/Strep

Sequence :

A2-K1292

Assay :

Structure :

Inquiry :

Symbol/Gene Name:  UBE2O

Alias:  (E3-independent) E2 ubiquitin-conjugating enzyme,E2/E3 hybrid ubiquitin-protein ligase UBE2O,Ubiquitin carrier protein O,Ubiquitin-conjugating enzyme E2 O,Ubiquitin-conjugating enzyme E2 of 230 kDa,Ubiquitin-conjugating enzyme E2-230K,Ubiquitin-protein ligase O

Uniprot: Q9C0C9

Background: E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853).

Construct: 8His-StrepII-TEV-(GG)-UBE2O(A2-K1292 end)

Expression Host: Insect cell

Purity: 90%

Formulation: 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol

Supplied as sterile

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20 to -80

Store it under sterile conditions at -20 to -80. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.