UBE2S-H00A

Family :

E2 ubiquitin-conjugating enzyme

Catalog No. :

UBE2S-H00A

Protein :

UBE2S

UniProt :

Q16763

Organism :

Homo sapiens

Expression system :

Insect cell

Tag :

No tag

Sequence :

N2-L222

Assay :

Structure :

Inquiry :

Symbol/Gene Name:  UBE2S

Alias:  Ubiquitin-conjugating enzyme E2 S,E2 ubiquitin-conjugating enzyme S,E2-EPF,Ubiquitin carrier protein S,Ubiquitin-conjugating enzyme E2-24 kDa,Ubiquitin-conjugating enzyme E2-EPF5,Ubiquitin-protein ligase S

Uniprot: Q16763

Background: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:22496338). Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit (PubMed:19820702, PubMed:19822757, PubMed:27259151). Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A (PubMed:16819549). In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination (PubMed:20061386, PubMed:20622874).

Construct: (GG)-UBE2S(N2-L222 end)

Expression Host: Insect cell

Purity: 90%

Formulation: 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 1 mM DTT, 5% glycerol.

Supplied as sterile

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20 to -80

Store it under sterile conditions at -20 to -80. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.