UBE2T-H00A

Family :

E2 ubiquitin-conjugating enzyme

Catalog No. :

UBE2T-H00A

Protein :

UBE2T

UniProt :

Q9NPD8

Organism :

Homo sapiens

Expression system :

Insect cell

Tag :

No tag

Sequence :

Q2-V197

Assay :

Structure :

Inquiry :

Symbol/Gene Name:  UBE2T

Alias:  Ubiquitin-conjugating enzyme E2 T,Cell proliferation-inducing gene 50 protein,E2 ubiquitin-conjugating enzyme T,Ubiquitin carrier protein T,Ubiquitin-protein ligase T

Uniprot: Q9NPD8

Background: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair. Acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway (PubMed:16916645, PubMed:17938197, PubMed:19111657, PubMed:19589784, PubMed:28437106). Also mediates monoubiquitination of FANCL and FANCI (PubMed:16916645, PubMed:17938197, PubMed:19111657, PubMed:19589784). May contribute to ubiquitination and degradation of BRCA1 (PubMed:19887602). In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination (PubMed:20061386).

Construct: (GG)-UBE2T(Q2-V197 end)

Expression Host: Insect cell

Purity: 80%

Formulation: 50mM Tris-HCl (pH 7.5), 200mM NaCl, 20% glycerol, 1mM DTT.

Supplied as sterile

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20 to -80

Store it under sterile conditions at -20 to -80. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.