UHRF2-H00A

Family :

E3 ubiquitin-protein ligase

Catalog No. :

UHRF2-H00A

Protein :

UHRF2

UniProt :

Q96PU4

Organism :

Homo sapiens

Expression system :

E. coli

Tag :

No tag

Sequence :

T419-K648

Assay :

Structure :

Inquiry :

Symbol/Gene Name:  UHRF2

Alias:  E3 ubiquitin-protein ligase UHRF2,Np95/ICBP90-like RING finger protein,Np95-like RING finger protein,Nuclear protein 97,Nuclear zinc finger protein Np97,RING finger protein 107,RING-type E3 ubiquitin transferase UHRF2,Ubiquitin-like PHD and RING finger domain-containing protein 2,Ubiquitin-like-containing PHD and RING finger domains protein 2

Uniprot: Q96PU4

Background: E3 ubiquitin ligase that plays important roles in DNA methylation, histone modifications, cell cycle and DNA repair (PubMed:15178429, PubMed:29506131, PubMed:27743347, PubMed:23404503). Acts as a specific reader for 5-hydroxymethylcytosine (5hmC) and thereby recruits various substrates to these sites to ubiquitinate them (PubMed:27129234, PubMed:24813944). This activity also allows the maintenance of 5mC levels at specific genomic loci and regulates neuron-related gene expression (By similarity). Participates in cell cycle regulation by ubiquitinating cyclins CCND1 and CCNE1 and thereby inducing G1 arrest (PubMed:15178429, PubMed:15361834, PubMed:21952639). Ubiquitinates also PCNP leading to its degradation by the proteasome (PubMed:14741369, PubMed:12176013). Plays an active role in DNA damage repair by ubiquitinating p21/CDKN1A leading to its proteosomal degradation (PubMed:29923055). Promotes also DNA repair by acting as an interstrand cross-links (ICLs) sensor. Mechanistically, cooperates with UHRF1 to ensure recruitment of FANCD2 to ICLs, leading to FANCD2 monoubiquitination and subsequent activation (PubMed:30335751). Contributes to UV-induced DNA damage response by physically interacting with ATR in response to irradiation, thereby promoting ATR activation (PubMed:33848395).

Construct: UHRF2(SRA DomainT419-K648)

Expression Host: E. coli

Purity: 85%

Formulation: 50 mM Tris-HCl (pH 7.5), 200 mM NaCl, 20% glycerol, 1 mM DTT.

Supplied as sterile

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20 to -80

Store it under sterile conditions at -20 to -80. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.