USP5-H01A

Family :

DUBs

Catalog No. :

USP5-H01A

Protein :

USP5

UniProt :

P45974

Organism :

Homo sapiens

Expression system :

E. coli

Tag :

His

Sequence :

A2-S858

Assay :

Structure :

Inquiry :

Symbol/Gene Name:  USP5

Alias:  Ubiquitin carboxyl-terminal hydrolase 5,Deubiquitinating enzyme 5,Isopeptidase T,Ubiquitin thioesterase 5,Ubiquitin-specific-processing protease 5

Uniprot: P45974

Background: Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

Construct: 6His-TEV-GG-USP5(A2-S858 end)

Expression Host: E. coli

Purity: 90%

Formulation: 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol

Supplied as sterile

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20 to -80

Store it under sterile conditions at -20 to -80. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.