USP13-H00A

Family :

DUBs

Catalog No. :

USP13-H00A

Protein :

USP13

UniProt :

Q92995

Organism :

Homo sapiens

Expression system :

E. coli

Tag :

No tag

Sequence :

Q2-S863

Assay :

Structure :

Inquiry :

Symbol/Gene Name:  USP13

Alias:  Ubiquitin carboxyl-terminal hydrolase 13,Deubiquitinating enzyme 13,Isopeptidase T-3,ISOT-3,Ubiquitin thioesterase 13,Ubiquitin-specific-processing protease 13

Uniprot: Q92995

Background: Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Also regulates ERAD through the deubiquitination of UBL4A a component of the BAG6/BAT3 complex. Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data.

Construct: GG-USP13(Q2-S863 end)

Expression Host: E. coli

Purity: 80%

Formulation: 50 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM DTT, 5% glycerol.

Supplied as sterile

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20 to -80

Store it under sterile conditions at -20 to -80. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.