USP30-H01A

Family :

DUBs

Catalog No. :

USP30-H01A

Protein :

USP30

UniProt :

Q70CQ3

Organism :

Homo sapiens

Expression system :

Insect cell

Tag :

His

Sequence :

T57-E517

Assay :

Structure :

Inquiry :

Symbol/Gene Name:  USP30

Alias:  Ubiquitin carboxyl-terminal hydrolase 30,Deubiquitinating enzyme 30,Ubiquitin thioesterase 30,Ubiquitin-specific-processing protease 30,Ub-specific protease 30

Uniprot: Q70CQ3

Background: Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy (PubMed:18287522, PubMed:24896179, PubMed:25527291, PubMed:25621951). Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate in mitophagic signaling (PubMed:25621951). Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65' (PubMed:25527291). Acts as negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2 (By similarity).

Construct: 8His-TEV-GG-USP30(T57-E517 end)

Expression Host: Insect cell

Purity: 90%

Formulation: 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol

Supplied as sterile

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20 to -80

Store it under sterile conditions at -20 to -80. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.