Wee1-H00A
Family ：
Kinase
Catalog No. ：
Wee1-H00A
Protein ：
Wee1
UniProt ：
P30291
Organism ：
Homo sapiens
Expression system ：
Insect cell
Tag ：
No tag
Sequence ：
M291-K575
Assay ：
Structure ：
Inquiry ：
是
Symbol/Gene Name: WEE1
Alias: Wee1-like protein kinase,WEE1hu,Wee1A kinase
Uniprot: P30291
Background: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Construct: (G)-Wee1(M291-K575)
Expression Host: Insect cell
Purity: 90%
Formulation: 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
Supplied as sterile
Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Endotoxin:
< 0.01 EU per μg of the protein
Shipping:
Liquid. It is shipped out with blue ice.
Stability & Storage:
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Recombinant protein
CONTACT US
Telephone：(781)201-9739
Adresse：Block A5, 6 Dongsheng West Road Jiangyin, Jiangsu Province 214437 People’s Republic of China
E-mail：info@wuxibiortus.com
FOLLOW US
Copyright © 2020 Wuxi Biortus Biosciences Science Co., Ltd 苏ICP备15024653号-1 Power by www.300.cn