ITK-H00A
Family ：
Kinase
Catalog No. ：
ITK-H00A
Protein ：
ITK
UniProt ：
Q08881
Organism ：
Homo sapiens
Expression system ：
Insect cell
Tag ：
No tag
Sequence ：
M1-L620
Assay ：
Structure ：
Inquiry ：
是
Symbol/Gene Name: ITK
Alias: Tyrosine-protein kinase ITK/TSK,Interleukin-2-inducible T-cell kinase,IL-2-inducible T-cell kinase,Kinase EMT,T-cell-specific kinase,Tyrosine-protein kinase Lyk
Uniprot: Q08881
Background: Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation (PubMed:12186560, PubMed:12682224, PubMed:21725281). Required for TCR-mediated calcium response in gamma-delta T-cells, may also be involved in the modulation of the transcriptomic signature in the Vgamma2-positive subset of immature gamma-delta T-cells (By similarity). Phosphorylates TBX21 at 'Tyr-530' and mediates its interaction with GATA3 (By similarity).
Construct: ITK(M1-L620 end)
Expression Host: Insect cell
Purity: 90%
Formulation: 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
Supplied as sterile
Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Endotoxin:
< 0.01 EU per μg of the protein
Shipping:
Liquid. It is shipped out with blue ice.
Stability & Storage:
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Recombinant protein
CONTACT US
Telephone：(781)201-9739
Adresse：Block A5, 6 Dongsheng West Road Jiangyin, Jiangsu Province 214437 People’s Republic of China
E-mail：info@wuxibiortus.com
NEWS FEEDS
FOLLOW US
Copyright © 2020 Wuxi Biortus Biosciences Science Co., Ltd 苏ICP备15024653号-1 Power by www.300.cn