tCypD-H01A

Family :

Protease and Enzyme

Catalog No. :

tCypD-H01A

Protein :

tCypD

UniProt :

P30405

Organism :

Homo sapiens

Expression system :

E. coli

Tag :

His

Sequence :

S43-S207

Assay :

Structure :

Inquiry :

Symbol/Gene Name:  PPIF

Alias:  Peptidyl-prolyl cis-trans isomerase F, mitochondrial,PPIase F,Cyclophilin D,CyP-D,CypD,Cyclophilin F,Mitochondrial cyclophilin,CyP-M,Rotamase F

Uniprot: P30405

Background: PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:20676357). Involved in regulation of the mitochondrial permeability transition pore (mPTP) (PubMed:26387735). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated (PubMed:26387735). In cooperation with mitochondrial p53/TP53 is involved in activating oxidative stress-induced necrosis (PubMed:22726440). Involved in modulation of mitochondrial membrane F(1)F(0) ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels (By similarity). Has anti-apoptotic activity independently of mPTP and in cooperation with BCL2 inhibits cytochrome c-dependent apoptosis (PubMed:19228691).

Construct: 6His-TEV-tCypD (S43-S207) (K175I)

Expression Host: E. coli

Purity: 90%

Formulation: 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol

Supplied as sterile

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20 to -80

Store it under sterile conditions at -20 to -80. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.