Family :

Protease and Enzyme

Catalog No. :

ido-O41A

Protein :

ido

UniProt :

E2GIN1

Organism :

Bacillus thuringiensis

Expression system :

E. coli

Tag :

Flag/His

Sequence :

M1-K240

Assay :

Structure :

Inquiry :

Symbol/Gene Name:  ido

Alias:  L-isoleucine-4-hydroxylase,L-isoleucine dioxygenase,IDO

Uniprot: E2GIN1

Background: Catalyzes the hydroxylation of L-isoleucine to produce (4S)-4-hydroxy-L-isoleucine (PubMed:20665018, PubMed:19850012, PubMed:21821743). Can also catalyze the hydroxylation of L-leucine, L-norvaline, L-norleucine and L-allo-isoleucine, as well as the sulfoxidation of L-methionine, L-ethionine, S-methyl-L-cysteine, S-ethyl-L-cysteine, and S-allyl-L-cysteine (PubMed:21821743).

Construct: Flag-6His-TEV-G-ido(M1-K240 end)

Expression Host: E. coli

Purity: 90%

Formulation: 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol

Supplied as sterile

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20 to -80

Store it under sterile conditions at -20 to -80. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.