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Instrumentations

Instrumentations

  • Categories:Assays
  • Time of issue:2020-08-14 00:00:00
  • Views:0
Description:Compound screening against a drug target whether by high-throughput screening or fragment screening is a vital step in early stage drug discovery. Biortus has an extensive collection of plate readers (detecting UV/light absorbance, fluorescence, luminescence, and scintillation) that allows development of many kinds of biochemical assays including cell-based assays. Coupled with our automated liquid handling machines, we could perform various high-throughput screening (HTS) for our clients. In addition, Biortus currently holds two other specialised screening technologies, Mobility Shift Assays (Caliper EZ Reader II, great for monitoring enzyme reactions) and Surface Plasmon Resonance (SPR, Biacore S200) for fragment screening. Below is a non-exhaustive list of assays performed in Biortus:
Description:Compound screening against a drug target whether by high-throughput screening or fragment screening is a vital step in early stage drug discovery. Biortus has an extensive collection of plate readers (detecting UV/light absorbance, fluorescence, luminescence, and scintillation) that allows development of many kinds of biochemical assays including cell-based assays. Coupled with our automated liquid handling machines, we could perform various high-throughput screening (HTS) for our clients. In addition, Biortus currently holds two other specialised screening technologies, Mobility Shift Assays (Caliper EZ Reader II, great for monitoring enzyme reactions) and Surface Plasmon Resonance (SPR, Biacore S200) for fragment screening. Below is a non-exhaustive list of assays performed in Biortus:
Information

Compound screening against a drug target whether by high-throughput screening or fragment screening is a vital step in early stage drug discovery. Biortus has an extensive collection of plate readers (detecting UV/light absorbance, fluorescence, luminescence, and scintillation) that allows development of many kinds of biochemical assays including cell-based assays. Coupled with our automated liquid handling machines, we could perform various high-throughput screening (HTS) for our clients. In addition, Biortus currently holds two other specialised screening technologies, Mobility Shift Assays (Caliper EZ Reader II, great for monitoring enzyme reactions) and Surface Plasmon Resonance (SPR, Biacore S200) for fragment screening. Below is a non-exhaustive list of assays performed in Biortus:

 

Assay Description

Mode of Detection

Instrument

Feature Highlights

Kinase Assays

Mobility Shift and Fluorescence

Caliper EZ Reader II

  • Real Time Kinetic Analysis
  • Selectivity Profiling

ADP-Glo Assay

Luminescence

TECAN Infinite F200 Pro/M1000
 

 

Perkin Elmer Envision 2104
 

  • Multifunctional Plate Reader (Fluorescence, Fluorescence Polarisation, Time-Resolved Fluorescence, Absorbance and Luminescence)

LANCE TR-FRET

Time-Resolved Fluorescence

Fluorescence Polarization Assay

Fluorescence Polarization

CellTiter-Glo® Luminescent Cell Viability Assay

Cell-Based Luminescence

MTT Cell Proliferation and Cytotoxicity Assay

Cell-Based Absorbance

AlphaScreen

Chemical Energy Transfer and Fluorescence

Scintillation Proximity Assay

Radiometric

Perkin Elmer TopCount NTX HTS
 

  • Ideal for Detecting Protein-Protein Interaction

Surface Plasmon Resonance

Light Absorption Shift

Biacore S200

  • Sensitive Binding Detection for Fragment Screening
  • Real Time Analysis
  • Provides Kon and Koff

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